ZFIN Image: Hou et al., 2016, Fig. 4

Image

Figure Caption

Fig. 4

Emergency monopoiesis occurs upon intravenous E. coli (5?10?�?10³ cfu) infection.

(A), WISH of mfap4 (red arrows) in the CHT of a larva treated with PBS or E. coli at 2 dpi (4 dpf). (B), The data obtained for mfap4⁺ macrophages at different time points after challenge (36.13?�?4.52 vs 53.88?�?3.90; 64.25?�?6.58 vs 57.00?�?3.29; 160.44?�?9.92 vs 53.20?�?1.90; 87.00?�?15.67 vs 42.44?�?4.21; 24.71?�?2.14 vs 23.00?�?2.30 in E. coli vs PBS group at each time point. N???8 in each group). (C,D), Fluorescence images (C) and calculation (D) of mpeg1-GFP⁺ macrophages in PBS (25.50?�?2.94; N?=?8) or E. coli (13.13?�?1.09; N?=?8) treated larval CHT at 6 hpi. The red signals indicate the bacteria phagocytosed by mpeg1-GFP⁺ macrophages (white arrows). (E), The percentage of mpeg1-GFP⁺ macrophages that co-stained with TUNEL at 6 hpi (21.13?�?3.86 vs 2.82?�?1.05 in E. coli vs PBS group. N???10 in each group). (F), Time-lapse imaging of an infected Tg(mpeg1:eGFP) CHT from 1 dpi to 1.5 dpi (see also Video S5). (G-H), WISH (G) and calculation data (H) for mfap4⁺ macrophages (red arrows) in the runx1 mutant treated with either PBS or E. coli at 2 dpi (4 dpf) (WT: 117.9?�?9.63 vs 25.38?�?0.96 E. coli vs PBS; runx1 mut: 110.3?�?6.88 vs 44.40?�?5.72 E. coli vs PBS; N???5 in each group). Scale bars, 20??m.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.