ZFIN Image: Hou et al., 2016, Fig. 1

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Figure Caption

Fig. 1

Bacterial (E. coli) phagocytosis by both macrophages and neutrophils following intravenous injection.

(A), The intravenous injection site (red arrow) of E. coli (5?10?�?10³ cfu) in 2 dpf embryo. Scale bars, 200??m. (B), The numbers of Dsred⁺ E. coli foci in the CHT regions at different time points after injection (13.90?�?1.31; 16.20?�?2.35; 11.90?�?1.33; 4.60?�?0.65; 2.20?�?0.33; 1.40?�?0.40; 0.60?�?0.22 at each time points; 10 embryos were counted in each group). (C), The percentage of macrophages (72.06?�?4.44, N?=?8) and neutrophils (36.93?�?8.89, N?=?8) involved in bacterial phagocytosis at 6 hpi in the CHT. (D,E), Time-lapse imaging of an infected Tg(mpeg1:eGFP) (D) or Tg(lyz:eGFP) (E) CHT from 0.5 hpi to 6 hpi. The white stars in (D) denote mpeg1-GFP⁺ macrophages that engulfed large amounts of Dsred⁺ E. coli. The white arrowheads in (E) indicate the initial aggregation of Dsred⁺ E. coli on the surface of lyz-GFP⁺ neutrophils, which were quickly phagocytosed. The red foci in (D,E) represent the phagocytosed bacteria. Scale bars, 20??m. See also Video S1 and S2.

Acknowledgments

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