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Fig. 7
ELMO1 knockout, but not hyperglycaemia, causes an increase in apoptotic cells within the zebrafish embryo as well as the pronephros, which can be rescued with pancaspase inhibitor, zVAD-fmk.
(A) TUNEL assay was carried out on Co. CRISPR injected Tg(wt1b:EGFP) embryos at 48 hpf. The embryos showed no incidence of apoptosizing cells (indicated with the red spots) within the renal structure. (B) ELMO1 CRISPR injected embryos showed a strong incidence of apoptosizing cells (red spots) universally as well as within the renal structure. (C) Indicated that hyperglycaemic embryos do not display increased apoptosis within the pronephros and (D) indicates that ELMO1 crispants displayed normalized renal structure and a drastic reduction of apoptosis, upon zVAD-fmk treatment, which is quantified in the graph. (E) The white line in (D) indicates a scale bar representing 100 μm, the white arrow head indicated an enlarged glomerulus and the white asterisk illustrates shortening of the glomerular neck of the ELMO1 crispants as compared to the control. The white dotted circle encloses the glomerulus of the zebrafish pronephros. The number of embryos per condition analysed are 11.