ZFIN Image: Hoodless et al., 2016, Fig. 2

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Figure Caption

Fig. 2

Treatment with AT7519 or flavopiridol accelerates resolution of neutrophilic inflammation.

Tg(mpx:EGFP)ⁱ¹¹⁴ zebrafish embryos underwent tailfin transection at 3?dpf and were serially imaged at various time points post-injury. (a) Embryos were micro-injected with DMSO or (b) AT7519 at 4?hpi with representative images (80x magnification) from 4?h [i] and 24?h [ii] shown. (c [i]) Serial neutrophil numbers at each tailfin were quantified with the blue arrow indicating the 4?hpi time of drug administration. ?40 fish from 4 independent experiments. Data expressed as ±SEM. (c [ii]) In separate experiments, Tg(mpx:EGFP)ⁱ¹¹⁴ zebrafish embryos were also treated with flavopiridol (FVP) at 4?hpi and the neutrophil numbers at the tailfin quantified. ?31 fish from 3 independent experiments. (d) The total neutrophils in the entire embryo at 24?hpi and DMSO/AT7519/FVP treatment were imaged and quantified. ?10 fish from 3 independent experiments. (e) Time lapse movies of embryos (3 per group) were analysed and the number of cells which migrate to the wound for 15?hpi and DMSO/AT7519 treatment was counted. *p?<?0.05 at 24?hpi, data analysed by two-way ANOVA then post-test Newman-Keuls or unpaired t-test. n/s: not significant.

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Acknowledgments

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