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Fig. 3
Otophylloside N attenuated PTZ-induced cell injury on primary cortical neurons. The neurons were treated with indicated concentrations of PTZ or PTZ + Otophylloside N (OtoN) for 24 h at 7 DIV. (A) Immunofluorescence detection of morphological changes of neurons and bright field of the same area. The cells were immunostained with neuronal anti-β-Tubulin marker (green) and nuclear DAPI tag (blue). (B) OtoN inhibited PTZ-induced toxicity in neurons. Cell viability is presented as a percentage of the control, and each value represents the mean ± SD of three independent experiments. (C) OtoN decreased PTZ-induced LDH intracellular efflux in neurons. The LDH efflux level is presented as a percentage of the control, and each value represents the mean ± SD of three independent experiments. ##P < 0.01 compared with the control. ∗∗P < 0.01 compared with the 30 mM PTZ treatment. Bar: 100 μm.