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Fig. 3 S1
Gene expression within the zebrafish and stickleback jaw joints.
(A–D) In situ hybridization reveals broad chondrocyte expression of prg4a but no enrichment within jaw joint articular chondrocytes (C–D, magnified views). (E) Zebrafish has3 is expressed in chondrocytes just underneath the articular surface (arrow) and in a small number of cells within the growth plate (arrowhead). (F) Expression of matn1 is excluded from superficial chondrocytes of the zebrafish jaw joint at 1 mpf. (G, H) Immunofluorescence staining for Col2a1 and Aggrecan protein reveals broad cartilage expression yet exclusion from jaw articular chondrocytes. n = 3 each panel. Scale bars, 50 μm.