Fig. 4
Ber, E6, and Her do not enhance regeneration rates after neomycin damage. Fish were treated with either natural compound only or natural compound and 200 μM neomycin in the presence of BrdU. Fish were counterstained with DAPI to identify neuromasts based on characteristic morphology, as shown in the inset where DAPI-labeled nuclei are blue and red nuclei are BrdU+. The line encircles the neuromast and the arrow denotes a BrdU+ supporting cell at the neuromast periphery. Scale bar = 10 μm. There is no difference in the number of BrdU+ cells per neuromast in fish that received neomycin + compound (gray bars) vs. those that received compound only (black bars; 2-way ANOVA, treatment effect F(1,67) = 1.882, p = 0.175). N = 8–10 and bars are ±SEM.