ZFIN Image: Leong et al., 2015, Fig. 7

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Fig. 7

FIGURE 7. Mecp2-mediated defects of axon projections leads to diminished sensory response. Representative images showing arrays of muscle fibers in WT control and Mecp2 morphants (KD) embryos immunostained with monoclonal antibody A4.1025 (myosin) (A). In all panels, anterior is to the left. (Blue-DAPI, red-myosin) Scale bar = 50 μm. Graphs showing rates of response upon tactile stimuli (red arrow in C) from the six experimental groups of WT and KD, including WT and KD coinjected with various rescue constructs indicated on the graph, upon tactile stimuli applied to the same region specified (B,C). Proposed model shows Mecp2 plays a part in primary sensory projections through regulating transcription of Sema5b and Robo2 (C). Each individual dot (B) represents individual embryos. N > 30. (^∗∗∗P < 0.0001, one-way ANOVA with Bonferroni’ post hoc test).

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Cell. Neurosci.