IMAGE

Fig. 2

ID
ZDB-IMAGE-171101-4
Source
Figures for Lauterbach et al., 2015
Image
Figure Caption

Fig. 2

Optical section in embryonic spinal cord of zebrafish obtained with HiLo microscopy.

(A) Lateral view of the spinal cord of transgenic zebrafish embryo expressing the fluorescent protein GCaMP5 pan-neuronally in Tg(elavl3:GCaMP5G) obtained with HiLo microscopy. (B) Same image obtained in UWF mode. The spatial distribution of GCaMP5G remains hidden under a haze of background fluorescence due to missing optical sectioning.Scale bars in both panels are 20 μm. (C) Corresponding z-stack in 3D visualization, imaged in HiLo mode. The pools of neurons on each side of the spinal cord are visible in two separate planes. (D) Same reconstruction in WF mode (grid step 10 μm/line). See also S1 Movie.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One
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