ZFIN Image: He et al., 2015, Fig. 5

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Figure Caption

Fig. 5

K287Q acetylation mimicking mutation affects Hsp90α1 function in thick filament organization.

DNA constructs expressing the myc-tagged K287R or K287Q mutant were co-injected with Hsp90α1 ATP-MO into fertilized eggs of zebrafish. The injected embryos were stained with anti-myc (9E10) and anti-MHC (F59) antibodies at 28 hpf. A-C. A mosaic pattern of rescue was detected in myofibers expressing the Hsp90α1 K287R mutαnt, mimicking the unacetylated lysine. D-F. K287Q mutant, mimicking the acetylated lysine state, failed to rescue the myofibril defect (D-F). G. Plot showing the number of rescued myofibers in 8–10 individual embryos injected with K287R, or K287Q mutant. Scale bar = 20 μm.

Acknowledgments

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