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Fig. 1
Expression of minimal promoters from mMTI, SV40, EF1α and CMV.
(A) The schematic drawing of pTRE/G2 vector used for testing promoter activity. (B and C) Representative images of embryos injected with pTRE/G2 vectors containing mMTI, SV40, EF1α or CMV minimal promoters under a fluorescence microscope at 24 and 48 hpf. (D) Expression of EGFP was detected by qRT-PCR in injected embryos at 24 and 48 hpf. Data were calculated from three independent experiments and given as means ± standard deviation. * and ** indicate p<0.05 and p<0.01 versus the group injected with pTRE/G2-mMTI embryos.
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