ZFIN Image: Vettori et al., 2017, Fig. S2

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Fig. S2

HRE transgene expression: Tg(4xhre-tata) lines have overlapping expression patterns. Conventional (A, A′, A′′, B, B′, B′′, C, C′, and C′′) and confocal (D, D′, D′′, E, E′, and E′′) lateral views of double transgenic Tg(4xhre-tata:eGFP)^ia21/Tg(4xhre-tata:mCherry,cmlc2:eGFP)^ia22 embryos. (D, D′, D′′, E, E′, and E′′) The 48-hpf embryos, with anterior to the left. (D–D′′) Head region of double transgenic embryo: D, green channel; D′, red channel; D′′, merge. (E–E′′) Tail region of double transgenic embryo: E, green channel; E′, red channel; E′′, merge. (Scale bar: 100 μm.) Comparisons between the two reporter lines revealed significant overlap in the expression profile of the 4xhre-tata transgene, suggesting the independence of the transgene expression from the genomic site of insertion. (Magnification: A–C, 8×.)

Acknowledgments

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