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Fig. 3 S1
Zebrafish primary cell culture.
Top row shows maximum projected images of cell clusters imaged in standard media (RI = 1.333, left) or refractive index adjusted media (RI = 1.362, right). The bottom row shows orthogonal optical sections at the positons indicated with the solid line in the top row. Overall, the images document a loss of resolution along the Z-axis (= increasing distance from the coverslip) in standard mounting media, which can be prevented by Iodixanol supplementation.
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