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Fig. 3
Purpurin labels bones in adult zebrafish and mice without significantly affecting bone density and mass. (A) Images of the purpurin-stained corselet and tail fin in an adult zebrafish. Scale bars: 100 μm. (B) Images of the purpurin-stained digital bone (i), spine (ii), rib (iii) and femur (iv) of a P3 mouse. Scale bars: 300 μm. (C) Three-dimensional bone reconstruction of micro-CT scans on the adult zebrafish treated with DMSO and purpurin for 3 weeks. Scale bars: 1 cm. (D) The statistical results of bone mineral density (BMD; n=12), body length (BL; n=12) and bone mass (BM; n=12). Values are means±s.e.m., with differences determined by Student's t-test. NS, not significant.