Fig. S1
- ID
- ZDB-IMAGE-171003-5
- Publication
- Lin et al., 2017 - Molecular physiology of the hypocalcemic action of fibroblast growth factor 23 in zebrafish (Danio rerio)
- All Figures
- Figures for Lin et al., 2017
|
Fig. S1
Specificity and effectiveness of fgf23 MO. Embryos were injected with fgf23 cRNA (with GFP fusion) (A), or coinjected with fgf23 MO and cRNA (B). A B fgf23 MO was used to inhibit translation of zebrafish FGF23. The specificity and effectiveness of the fgf23 MO was confirmed by co-injection with zebrafish fgf23 cRNA (fused to GFP). Zebrafish embryos injected with only cRNAs exhibited GFP signals (Figure 1A), confirming translation of fgf23 cRNA. On the other hand, embryos co-injected with fgf23 MO and fgf23 cRNA showed no green fluorescence (Figure 1B), indicating that the MO specifically and effectively knocked-down the translation of fgf23 mRNA.