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Fig. S1
Specificity and effectiveness of fgf23 MO. Embryos were injected with fgf23 cRNA (with GFP fusion) (A), or coinjected with fgf23 MO and cRNA (B). A B fgf23 MO was used to inhibit translation of zebrafish FGF23. The specificity and effectiveness of the fgf23 MO was confirmed by co-injection with zebrafish fgf23 cRNA (fused to GFP). Zebrafish embryos injected with only cRNAs exhibited GFP signals (Figure 1A), confirming translation of fgf23 cRNA. On the other hand, embryos co-injected with fgf23 MO and fgf23 cRNA showed no green fluorescence (Figure 1B), indicating that the MO specifically and effectively knocked-down the translation of fgf23 mRNA.