ZFIN Image: Gioia et al., 2017, Fig. 2

Image

Figure Caption

Fig. 2

Skeletal evaluation of Chi/+ larvae. (A) Fin fold angle is represented in red in 11 dpf fish. Scale bar: 1 mm. (B) Representative images of 5 dpf larvae WT and Chi/+ stained with calcein. The delay in vertebral centra ossification is evident. Scale bar: 250 µm. (C) Percentage of 11 dpf fish presenting calcein positive (mineralized) vertebrae. c: vertebral centrum. Grey: WT; white: Chi/+. (D) Alcian blue and alizarin red staining of WT and Chi/+ 11 dpf larvae in ventral and lateral orientation. 5th ceratobranchyal (5CB, arrowhead), cleithrum (CL, arrow) and notochord (NC, asterisk) are indicated. Scale bar: 200 µm. Delayed mineralization, represented by reduced alizarin red staining, is evident in the mutant larvae. (E) Percentage of 11 dpf WT and Chi/+ larvae presenting distinctive levels of mineralization in the 5CB (i), the CL (ii) and the NC (iii). White indicates beginning/no mineralization, grey indicates incomplete mineralization and black indicates complete mineralization.

Figure Data

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Hum. Mol. Genet.