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Fig. 6
Cytosolic antiviral signaling is activated in uhrf1 mutants. (A) Change of pTbk1 protein levels quantified in three clutches of 120 hpf uhrf1 mutants and their siblings and (B) a representative western blot. (C) Embryos treated with 1 µM BX795 from 48-120 hpf were assayed for immune gene expression at 120 hpf by RT-qPCR in at least three clutches. Relative percentage change in gene expression is shown in treated uhrf1 compared with 0.1% DMSO-treated uhrf1 embryos (n=4). (D) Immune gene expression in uhrf1 mutant embryos injected with sting morpholino (MO) compared with uninjected uhrf1 mutants (n=4). (E) Examination of the expression of a limited panel of immune genes before and after 500 µg/µl Foscarnet (Fos) treatment in uhrf1 mutants (n=8). A line connecting two dots represents one paired sample of siblings from a single clutch. Significance was determined by t-test (A) or paired t-test (C-E). (F) Assessment of macrophage number in the head of embryos treated with 500 µg/µl Fos. The area quantified in WT and uhrf1 mutants is indicated (red dashed box). Error bars represent s.d.