ZFIN Image: Sedykh et al., 2017, Fig. S1

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Figure Caption

Fig. S1

Generation and characterization of mutant alleles at zic2a and zic2b loci.

A: zic2a^gbt133 was identified in an insertional mutagenesis screen (Clark et al., 2011). This insertion in the first coding exon of zic2a, near the N-terminus of the predicted open reading frame of zic2a. This allele is predicted to be a functional null. Tracks from individual embryos deep-sequenced on the Illumina platform show that the insertional allele inhibits transcription of the first coding exon of zic2a. B: CRISPR and TALEN nucleases were designed to target different sites in the first coding exon of zic2b.Two mutant alleles of zic2b were generated using CRISPR mutagenesis (uw1127 and uw1116). A third allele of zic2b was generated using TALEN mutagenesis (uwt104). Uwt104 and uw1116 cause frameshifts; uw1127 is an in-frame insertion that contains a translational termination codon. All three alleles encode truncated proteins that do not contain the ultra-conserved zinc binding domains. C: normal 5 dpf sibling and D: zic2 mutant with cerebral edema (arrow) from a zic2 heterozygous carrier incross.

Figure Data

Phenotype details

Acknowledgments

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Reprinted from Developmental Biology, 429(1), Sedykh, I., Yoon, B., Roberson, L., Moskvin, O., Dewey, C.N., Grinblat, Y., Zebrafish zic2 controls formation of periocular neural crest and choroid fissure morphogenesis, 92-104, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.