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Fig. S8
Ptk2 morpholino targets the translation start site of the ptk2aa. (A) Design of the reporter construct (MOptk2 BS-GFP) that carries the ptk2aa 5′UTR carrying the translation start site fused to the open reading frame of GFP without the translation start site. This construct carries the SP6 promoter that enables in vitro transcription. (B-C) Live images of 1 dpf embryos injected with MOptk2 BS-GFP mRNA. (D-E) Live images of 1 dpf embryos injected with MOptk2 BS-GFP mRNA and ptk2a morpholino (MO-ptk2).
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Reprinted from Developmental Biology, 429(1), Kara, N., Wei, C., Commanday, A.C., Patton, J.G., miR-27 regulates chondrogenesis by suppressing Focal Adhesion Kinase during pharyngeal arch development, 321-334, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.