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Fig. S4
Conditional and neural crest-specific miR27 overexpression does not lead to any defects in pharyngeal arch morphogenesis. (A) Alcian blue staining of heat shocked Tg(hsp70:miR-27b-eGFP) and wild-type siblings. Heat shock was performed at 24 hpf and embryos were fixed and stained at 4dpf. Images are ventral views of the head cartilage and the ratio of the observed phenotype to the total number of embryos is shown for each image. (B) qRT-PCR of miR-27b in 72hpf old Tg(hsp70:miR-27b-eGFP) and wild-type siblings. Relative miR-27b levels were normalized to control U6 snRNA.
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Reprinted from Developmental Biology, 429(1), Kara, N., Wei, C., Commanday, A.C., Patton, J.G., miR-27 regulates chondrogenesis by suppressing Focal Adhesion Kinase during pharyngeal arch development, 321-334, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.