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Fig. 5
CRIPTO knockdown reduces invasion and tumor growth of human PCa cells in vivo. (a) PC-3M-Pro4Luc2_dTomato human PCa cells with scrambled shRNA control (NT) and shCRIPTO no. 2 shRNAs were injected into the duct of Cuvier to monitor extravasation and formation of distant growth in vivo (30 embryos injected per group). (b) CRIPTO knockdown reduces whole-body tumor burden at 4?dpi (days post injection). Error barsąs.e.m. (c) CRIPTO knockdown reduces the number of extravasated cells at 1 and 4?dpi at the caudal hematopoietic tissue. Error barsąs.e.m. **P<0.01 and ***P<0.001.
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