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Fig. 3
Increased cx43 at the time of joint formation reduces the frequency of evx1-positive rays. A: Whole-mount ISH shows increased cx43 and decreased evx1 expression at the time of joint initiation (87 hpa) in the Tg(hsp70:miR-133sp)pd48 line following a heat pulse at 72 hpa. Transgene-positive fins, sp(+) (n?=?9 fins), were compared to nontransgenic sp(-) siblings (n?=?7 fins). B: The sp(+) transgenic fish showed reduced frequency of evx1 expression compared to the sp(-) nontransgenic sibling. Both sp(+) and sp(-) fish were similarly treated for heat shock. Statistically significant differences were determined by the Student's t-test, where P?<?0.01 (**). Error bars represent the standard deviation.