Fig. S8

Fig. S8

In situ hybridization analysis of expression of different fbrsl1 transcript isoforms.

A) Schemic presentation of fbrsl1 isoforms. The positions of riboprobes are shown as red lines. Non­coding and coding parts of exons are shown as white and dark blue rectangles, respectively. Exons 9a and 9b are mutually exclusive exons. (8-C) left panels, whole mount in situ hybridization with isoform­specific riboprobes, TSS1 (B) and TSS2 (C) in wild type 24 hpf embryos. Right panels, a flat mount preparation of embryos showing in left panels; yolk and eyes were removed. lsoform-specific sense and antisense riboprobes were designed against alternative first exons 1A (TSS1, 883-bp long probe comprising 849 bp of 5'UTR and 34 bp of coding region) and exon 1 B (probe TSS2, 652 bp). Arrow points hypothalamus, arrowhead points ventral diencephalon and asterisk points telencephalon.