Fig. S10

Fig. S10

Loss of isl2 compensates for increased venous markers caused by loss of notch signaling in rbpsuh morphants. (A-P) Representative images showing the expression of arterial and venous markers in wild type, isl2^ATG MO, rbpsuh^MO and rbpsuh^MO + isl2^{ATG MO} at 24 hpf. Compared to wild type controls (A, E, I, M), expression of the venous markers flt4 (B) and ephB4 (F) is reduced in the trunk of isl2 morphants while there is no obvious change in the expression of arterial markers notch3 (I) and ephrinb2 (N). In rbpsuh^MO, expression of the venous markers flt4 (C) and ephB4 (G) is increased and there is a decrease in the expression of arterial markers notch3 (K) and ephrinb2 (O). In rbpsuh^MO + isl2^{ATG MO} double knockdown expression of venous markers (D, H) is similar to wild type; however, the level of arterial markers (L, P) remains the same as rbpsuh^MO. Scale bars are 50 m in A-P.