ZFIN Image: Xie et al., 2016, Fig. 7

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Fig. 7

In Gα₁₃ morphants, Fn fibrils in the regions in which endocardial and myocardial precursors reside are disrupted. Whole-mount Fn immunostaining was performed in control (A-E, n=6) and gna13a/b MO-injected (A′-E′, n=7) Tg(nkx2.5: ZsYellow/sox17: memCherry) embryos at 13 s (A, A′) Projections of XY views of confocal Z-stacks spanning the left-hand side of the embryo, with labeled myocardial precursors (blue), endoderm (magenta), and Fn fibrils (green). (B, B′) A single Z-plane from Z-stacks in A, A′. (C-E, C′-E′) Images of XZ transverse sections (corresponding colored boxes on the far right corners) of the regions indicated by horizontal pink, yellow and cyan lines in A, A′. Pink dots: presumptive endocardial cell populations; yellow dots: myocardial cell populations; pink arrowheads: leading regions of myocardial populations; yellow arrowheads: regions between endocardial and myocardial precursors; yellow dashed line: midline; D: dorsal; V: ventral. Scale bars: 20 µm.

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Acknowledgments

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Reprinted from Developmental Biology, 414, Xie, H., Ye, D., Sepich, D., Lin, F., S1pr2/Gα13 signaling regulates the migration of endocardial precursors by controlling endoderm convergence, 228-43, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.