ZFIN Image: Duboué et al., 2017, Fig. S4

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Figure Caption

Fig. S4

Increase in dHb neuronal activation post-shock; related to Figure 4

(A) Live imaging of 8 day Tg(gng8:gal4);Tg(UAS:GCaMP7a) larva paralyzed with rhodamine conjugated a-bungarotoxin. Dorsal view, anterior to the left. Left panel shows GCaMP7a labeling, middle panel is rhodamine labeling and right panel is merged image. Background fluorescence was masked using Fiji and Matlab. (B) Histogram of log fold change in GCaMP7a activation (ln) of 4195 neurons post-shock, with corresponding probability density function (solid red line). Mean and standard deviation indicated by vertical dashed lines. Neurons were defined as responsive when their fold change in activity exceeded 1 standard deviation. (C) Representative traces of fluorescence (ΔF/F) for responsive (red) and nonresponsive neurons (black). (D) Representative traces of responsive neurons with firing events (blue arrowheads) identified using the findpeaks function in MATLAB. (E) Distribution of timing to first firing event post-shock for responsive neurons in the dHb of situs solitus larvae, and those with left or right isomerized dHb.

Acknowledgments

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