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Fig. S2
Increased freezing after shock in larvae with bilaterally severed FR; related to Figure 1
(A-B) Live confocal images of the Hb-IPN pathway in Tg(gng8:nfsB-GFP-CAAX) controls with lesions (white arrow heads) (A) lateral (n=10) or (B) medial (n=13) to the FR captured at 3 d before and after laser exposure, and 5 day later following behavioral testing. Scale bar is 50 μm. (C) Total distance traveled significantly decreased post-shock in larvae with bilaterally severed FR (n=15; Kruskal-Wallis ANOVA H=27.3; p<0.0001; Dunn’s post test, p<0.05) compared to uncut controls (n=13) or those with lesions medial (n=13) or lateral (n=10) to the FR. (D) Freezing was also significantly increased (Kruskal-Wallis ANOVA H=28.22, p<0.0001; Dunn’s post-test, p<0.05). Horizontal bars superimposed over scatter plots of individual larvae (circles) represent mean ±SEM for 1-min pre- and post-shock intervals. No significance (ns) and significance below p=0.05 (*) are denoted.