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Fig. 3
Fear Responses Are Diminished by dHb with Left Identity
Expression pattern of kctd12.1 in 7- to 8-day-old larvae with typical situs (situs solitus) and those with right- (red) or left- (blue) isomerized dHb (A). Following shock, larvae with right-isomerized dHb (n = 7) show a significant reduction in swimming (B) (Mann-Whitney U = 6, p < 0.01) and a significant increase in freezing duration (C) (Mann-Whitney U = 91, p < 0.001) and cortisol levels (D) (Mann-Whitney U = 0, p < 0.001) compared to controls (n = 9). Significant differences were not observed between larvae with left-isomerized dHb (n = 10) and their wild-type siblings (n = 30) for distance traveled (B, right) (Mann-Whitney U = 110, p = 0.221), freezing duration (C, right) (Mann-Whitney U = 71, p = 0.07), or cortisol levels (D, right) (averaged from five pools of 15 larvae; Mann-Whitney U = 10.5, p = 0.7302) post shock. Horizontal bars representing mean ± SEM are superimposed over scatterplots of individual larvae (circles). In (B) and (C), pre- and post-shock measurements correspond to 1-min intervals. Significance below p = 0.05 (∗) is noted. See also Figure S1.