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Fig. S4
A wave of TGFβ signaling is observed throughout the pharynx, Related to Figure 5.
(A-H) Spatiotemporal dynamics of TGFb signaling during PAA angioblast differentiation. Dorsal views of Tg(sox10):GFP) or Tg(sox17:GFP) embryos at the indicated developmental stages immunostained for GFP and pSMAD3 imaged in the green and far red channels to visualize neural crest (sox10) or endodermal pouches (sox17) (pseudocolored magenta) and cells experiencing active TGFβ signaling (pseudocolored yellow). 32 hpf, n=3 embryos; 36 hpf, n=6 embryos. Scale bars = 25μm.
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