ZFIN Image: Wehner et al., 2017, Fig. 5

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Fig. 5

Knockdown of col12a1a/b inhibits functional axon regeneration. a Cartoon showing strategy used for systemic delivery of Vivo-Morpholino (MO), which acts as cell-permeable translation blocking anti-sense molecules. MOs against col12a1a and col12a1b were co-injected into the cardiac ventricle at 3 dpf, 4?h before lesion. b col12a1a/b MO injection strongly reduces Col XII immunoreactivity in the lesion site compared to control MO injected animals. (t-test: ***P?<?0.001). c col12a1a/b MO injection inhibits axon regeneration after lesion (Fischer?s exact test: ***P?<?0.001). d col12a1a/b MO injection interferes with functional recovery after lesion (two-way ANOVA: F_1,116?=?5.534, p?<?0.05; t-test: ***P?<?0.001). a?d Lateral views are shown (dorsal is up, rostral is left). BF: brightfield. n.s.: not significant. Scale bars: 200?�m c and 100?�m (b,c). Error bars indicate s.e.m

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Acknowledgments

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