ZFIN Image: Quillien et al., 2017, Fig. S3

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Figure Caption

Fig. S3

Reporter assays for putative endothelial enhancers, relating to Figure 5. (A) Proportion of cryaa:mcherry-positive embryos with endothelial Egfp expression following injection with indicated construct. (B) dusp5 locus. Black box indicates element used for reporter assay with preferential mapping of ATAC-Seq reads in GFP-plus, but not GFP-minus nuclei. Grey box indicates fragment of dusp5 promoter used for promoter assays. (C-J) Epifluorescent overlay images of green and red fluorescence in embryos injected with indicated reporter constructs. ?Enh-basP? ? indicated enhancer upstream of basal promoter; Prom ? promoter element from indicated gene; Enh/Prom ? composite element with enhancer and cognate promoter from indicated gene. Ratios in left bottom corner denote number of embryos with endothelial green fluorescents over the number of cryaa:mcherry expressing embryos. Scale bar is 250 ?m.

Acknowledgments

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