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Fig. S2
Medial dbx1b+ cells give rise to all dHb neurons. TgBAC(gng8:CAAX-GFP) labels the cell membranes of dHb neurons (green). (A) TgBAC(dbx1b:nls-dTomato) labels cells medial and ventral to the GFP labeled neurons. Dorsal view, 5 dpf. (B,C) Lineage tracing using Cre recombinase under control of the dbx1b promoter [TgBAC(dbx1b:Cre-mCherry)] and a floxed reporter line [Tg(β-actin:loxP-hmgb1-eCFP-loxP-H2B-mCherry)] labels neurons throughout the dHb with nuclear mCherry at (B) 5 dpf and (C) in adult brain sections. Scale bars are (A,B) 30 μm and (C) 50 μm.
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