ZFIN Image: Mesureur et al., 2017, Fig. S4

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Figure Caption

Fig. S4

Related to Fig 1E and 1F. Mtz-mediated ablation of macrophages prevents B. cenocepacia K56-2 multiplication.

Fluorescence overlay images (red and green filter) of two DMSO-treated (left panels) and two Mtz-treated (right panels) mpeg1/umn⁺ embryos imaged at 1, 3, 24, and 48 h after iv injection with ~50 CFU B. cenocepacia K56-2 harbouring pIN301 (green). Bacterial replication in control embryos resulted in macrophage cell death, seen as mCherry positive debris (>24 hpi) (see also S5F Fig). At 28 and 48 hpi individual fluorescence images (green filter) are shown below the overlay images. Drawing indicates imaged area, marking the embryo sac extension (green) prone to autofluorescence. Arrow head indicates mCherry positive macrophages in apoptosis.

Acknowledgments

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