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Fig. 3
Immune cells dynamics in cryoinjured hearts.
(A) Zebrafish and medaka heart sections at 6 hpci, 2 dpci and 7 dpci were stained with isolectin-B4 (IB4) for macrophages, and Mpx antibody for neutrophils. Positive cells, both in the injured area itself and within 100 μm of the injured area, were quantified (B; n = 3). Dotted lines delineate the injured area; scale bars, 200 μm. Macrophage numbers in medaka were always lower than those in zebrafish and this difference was especially pronounced at 7 pd. Neutrophil numbers appeared similar at 6 hpci, were higher in medaka at 2 dpci, and their clearance was delayed in medaka compared to zebrafish.