ZFIN Image: Stainier et al., 2017, Fig. 5

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Figure Caption

Fig. 5

Neutrophil clearance is delayed after macrophage pre-depletion.

Adult zebrafish were injected with PBS or clodronate liposomes 1 day before injury. Hearts were collected at 6 hpci (A), 2 dpci (A), 7 dpci (A and B), and 1 mpci (C and D) to examine macrophages by both IB4 staining and mpeg1:EGFP expression, and neutrophils by immunostaining for both Mpx and mpx:GFP expression. (A) Neutrophil dynamics at 6 hpci, 2 and 7 dpci. (B) Neutrophil clearance by macrophage phagocytosis was observed in PBS-exposed hearts at 7 dpci. (C) Neutrophils remained in the injured area of CL-exposed hearts at 1 mpci. (D) Neutrophil clearance by IB4- and mpeg1:EGFP-double positive macrophages was observed in CL-exposed hearts at 1 mpci. Dotted lines delineate injured area; scale bars, 200 μm (A), 50 μm (B), 100 μm (C), and 20 μm (D).

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