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Fig. 4 S2
Delayed macrophage recruitment in zebrafish compromises scar resolution.
Adult zebrafish were injected with PBS or clodronate liposomes 1 day before injury. Hearts were collected at 1 mpci, and cryosections were stained with AFOG. Healthy muscle stained in orange, fibrin in red, and collagen in blue. Sections with the largest scar area for each heart (delineated by dotted lines) were selected and quantified (Figure 4G, n = 5). Scale bars, 200 μm. Scar tissue in CL-exposed hearts was significantly larger than in controls at 1 mpci.
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