ZFIN Image: Pei et al., 2016, Fig. S6

Image

Figure Caption

Fig. S6

Inhibition on pyrimidine synthesis by leflunomide partially phenocopies the prps1a:prps1b mutant phenotypes.

(A) Leflunomide treatment of wild-type embryos leads to a dose-dependent reduction in melanocytes and eye size. Leflunomide concentrations are as labeled. Arrows point to small eye and melanocyte reduction in the embryo treated with 2 uM of leflunomide. (B) Dose-dependent reduction of retinal iridophores in leflunomide treated embryos. (C) Quantification of eye size reduction. Eye size is measured by Image J. A significant reduction in eye size is detected between leflunomide at 0 uM and1 uM (n=10, p<0.001), and between leflunomide at 1 uM and 2 uM (n=10, p<0.001). (D) Dose-dependent reduction of neuromast hair cells in leflunomide treated embryos. A significant reduction in neuromast hair cells is detected between leflunomide at 0 uM and 1 uM (n=9, p=0.03), and between leflunomide at 1 uM and 2 uM (n=9, p<0.001). Graphs show the mean and s. e. m.. The data shown were replicated in another independent experiment.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.