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Fig. 5
Overexpression of rasl11b decreases proliferation of RPCs. A–C: Confocal dorsal views of 18 somite rx3:GFP embryos co-labeled with pHH3. D: Increasing doses of rasl11b resulted in decreased proliferation within the GFP positive eye field (control 100%, N = 3, n = 30; 200 pg rasl11b 72.87% ± 4.25% N = 3, n = 38, ****P < 0.0001; 400 pg rasl11b 62.19% ± 4.01%, N = 3, n = 31, ****P < 0.0001; one-way ANOVA, multiple comparisons test; error bars indicate SEM). E–G: Overexpression of rasl11b results in smaller eyes at 48 hpf (eye diameter relative to head length; control 0.48 μm ± 0.005 μm N = 3, n = 30; 400 pg rasl11b 0.39 μm ± 0.01 μm, N = 3, n = 36; ****P < 0.0001; one-way ANOVA, multiple comparisons test; error bars indicate SEM).