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Fig. 2
Knockdown of dmrt2a affected the levels of dmrt2b, myhz-2 and myhz-5 mRNA in zebrafish embryos. (A) The fold changes of dmrt2b mRNA in Cont-MO and dmrt2a-MO injected embryos at 24 hpf by qRT-PCR. (B) qRT-PCR detection of the fold changes of myhz-2 mRNA and myhz-5 mRNA in Cont-MO and dmrt2a-MO injected embryos at 24 hpf. (C) Whole mount in situ hybridization of muscle maker genes myod (a,d), myhz-2 (b,e) and myhz-5 (c,f) in Cont-MO embryos (a–c) and dmrt2a-MO embryos (d-f) at 24 hpf. Transverse sections of trunk regions in the corresponding embryos were shown in the right bottom. The ratios of embryos with specific signals are indicated. (D) The fold changes of myhz-2 mRNA and myhz-5 mRNA in control and dmrt2a-mRNA injected embryos at 24 hpf by qRT-PCR. Error bars indicate mean ± SD, n = 3, 30 embryos for RT-PCR and 20 embryos for WISH in each experiment. Student's t-test was used for statistical analysis (*P < 0.05).
Reprinted from Gene, 625, Lu, C., Wu, J., Xiong, S., Zhang, X., Zhang, J., Mei, J., MicroRNA-203a regulates fast muscle differentiation by targeting dmrt2a in zebrafish embryos, 49-54, Copyright (2017) with permission from Elsevier. Full text @ Gene