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Fig. 2 S2
Sec14l3 can self-assemble into oligomers.
(A) Living imaging of calcium release upon Wnt stimulation in PC3 cells. PC3 cells were transfected with calcium indicator plasmid Rcamp1.07 and then starved for 400 ng/μl Wnt3a, Wnt5a, Wnt8a and Wnt11 stimulation individually. PBS serves as negative control. The arrow indicates the time point when Wnt is added. Scale bar, 10 μm. (B) Sec14l3 can be assembled into an oligomer in HEK293T cells. (C) Sec14l3 oligomer formation is mediated by its CARL-TRIO and GOLD domains.
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