ZFIN Image: Venero Galanternik et al., 2017, Fig. 8

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Figure Caption

Fig. 8

Zebrafish FGPs differentiate from the CVP endothelium.

(a) Model diagram showing the location of the CVP imaged during timelapse acquisition in a dorsal (top) and lateral (bottom) orientation.( b–g) Stills from time-lapse movies of cells emerging from the CVP at around 2.5–3 dpf in a Tg(mrc1a:eGFP);Tg(kdrl:mCherry) double transgenic animal. Mrc1a:eGFP vessels are depicted in gray for ease of visualization, Kdrl:mCherry vessels are magenta and emerging FGPs are green (yellow arrows and numbers 1,2). Mrc1a:eGFP-positive FGPs emerge as single cells and stay in contact with the vessels in the perivascular space. (h), Model diagram showing the location of the CVP imaged during timelapse acquisition in a dorsal (top) and lateral (bottom) orientation.( i–k) Emergence of FGPs from the CVP in Tg(mrc1a:eGFP);Tg(kdrl:nlsmCherry) double transgenic animal. Newly emerging FGPs (white, yellow arrows, numbers 1–2) express Kdrl:nlsmCherry as they detach from the CVP (yellow arrows in j and teal pseudocolor in k).

Acknowledgments

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