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Fig. S3
Twelve-month-old zebrafish neuromuscular junction (NMJ) and lateral white muscle structure.
(A) The 3D co-localization analyses of z-stacks covering the entire thickness of the lateral muscle sections of the mSod1 and control (Ctrl) fish did not reveal any significant differences in pre-synaptic (0.48 ± 0.03 μm2 vs 0.54 ± 0.05 μm2) or post-synaptic cluster size (1.89 ± 0.26 μm2 vs 2.36 ± 0.33 μm2), or in the fluorescence intensity of the pre-synaptic (28.34 ± 2.42 a.u. vs 25.56 ± 1.39 a.u.) or post-synaptic clusters (14.88 ± 2.16 a.u. vs 16.09 ± 3.16 a.u.). The columns indicate the mean values ± SEM of the indicated parameter in five Ctrl and six mSod1 adult zebrafish. The measures were statistically analysed using an unpaired Student t-test. (B) Representative electron microscopy images of the NMJ (upper panel) and muscle ultrastructure (lower panel) in Ctrl and mSod1 adults. M: mitochondria; SV: synaptic vesicles.
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