ZFIN Image: Lagos et al., 2017, Fig. 5

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Figure Caption

Fig. 5

Cumulative survival and immune response of adult zebrafish immunized with MVs and infected with F. noatunensis subsp. orientalis. (A) Kaplan-Meier representation of cumulative survival of adult zebrafish vaccinated with 20 μg MVs and infected with 10⁶ CFU F. noatunensis subsp. orientalis (P value, 0.001 [log rank test]). (B) Histological examination by hematoxylin-eosin (HE) staining of spleen from unvaccinated challenged fish 7 dpc (panel a) and MV-vaccinated fish challenged with F. noatunensis subsp. orientalis at 7 dpc (panel b). Arrowheads indicate granulomatous structures. Bars, 150 μm (magnification, ×10). (C and D) Transcriptional response of selected cellular markers of kidney from MV-vaccinated fish before challenge (C) and after challenge (D) as measured by RT-qPCR. Bars represent means ± standard deviations (SD) of relative expression levels compared to those for the control (PBS injected). Relative expression levels were normalized to the expression of eef1a1l1. Asterisks indicate significant upregulation (****, P < 0.0001; ***, P < 0.001).

Acknowledgments

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