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Fig. S1
Assessment of visceral adipose tissue volume, plasma TG and hepatic steatosis in overfed zebrafish. (a) Changes in visceral adipose tissue volume in non-DIO and DIO zebrafish. Non-DIO group: n = 14; DIO group: n = 29. (b) Changes in plasma triglyceride (TG) levels in non-DIO and DIO zebrafish. Non-DIO group: n = 14; DIO group: n = 29. (c) Oil red O staining of liver sections. Red droplets indicate neutral lipid staining. Original magnification: × 400. Scale bar = 50 μm. Values are means ± SE. Statistical analyses were performed using Student’s t-test to compare the non-DIO and DIO groups at each time-point. *P < 0.05, **P < 0.01.