Fig. 1
fgf8a Gene Regulation during Retina Regeneration
(A) RT-PCR analysis of fgf8a, fgf8b, and Fgf-responsive genes in uninjured and needle-poke-injured retinas.
(B) In situ hybridization assays and BrdU immunofluorescence for fgf8a expression and MG proliferation, respectively, before and after injury to central retina in 6-month-old fish. Arrowheads point to fgf8a RNA in uninjured retina; arrows point to fgf8a RNA enriched in proliferating MG-derived progenitors at 4 dpi. Asterisk indicates injury site. Scale bar, 50 μm.
(C) RT-PCR analysis of ascl1, fgf8a, and gapdh RNAs in GFP+ MGs and GFP− non-MGs (retinal neurons) in uninjured and injured retinas that were FACS purified from gfap:GFP transgenic fish retinas.
See also Figure S1.