ZFIN Image: Chow et al., 2017, Fig. S1

Image

Figure Caption

Fig. S1

Co-localization of eGFP-GlyR1 and gephyrin. Related to Figure 1.

(A) Sections of spinal cord in zebrafish larvae stochastically expressing eGFP-GlyR?1 in motoneurons co-stained with anti-GFP (top) and anti- Gephryin (middle) show co-localization of GFP positive puncta with gephyrin positive regions (bottom).

(B) Quantification of the co-localization data. Mander?s Co-localization Coefficient (MCC), which quantifies the percentage of green fluorescence that has an above-threshold signal for gephyrin fluorescence, has a mean of 0.73. Pearson?s Correlation Coefficient (PCC) quantifies the correlation between the intensity of fluorescence in the gephyrin and GFP channels for synaptic ROIs and had a mean of 0.35. All cells had significant correlation of mean fluorescence intensity in synaptic ROIs between anti-GFP and anti-gephyrin staining.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Curr. Biol.