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Fig. 7
Genoarchitectonic signatures of the Rhombencephalo-Spinal primordium (RhSp).
(A-C′′). Hox1, Hox3, and Hox6 were expressed in the alar and basal plates of some caudal domains of the RhSp region in a sequential rostro-caudal order. (D-D′′) Nkx6 was detected at different degrees of expression mainly at the floor plate of the Di-Mesencephalic primordium (DiMes) and RhSp domains and a localized bilateral spot at the equivalent position of the fifth somite. (E-E´´) FoxB mRNA was observed extending caudally from the DiMes/RhSp border into the basal plate of the entire RhSp region. (F-G´´) Pax2/5/8 and Msx mRNAs were detected in some patches in the alar plate of the RhSp region. Expression patterns correspond to lateral (A-G) or dorsal views (A′-G′) at the 21 h post fertilization (hpf) embryonic stage, and are represented in schematics dorsal views (A′′-G′′). Somites (dotted lines) were used as main landmarks to localize the position of the patterns analyzed in the late neural plate. Scale bar: 50 μm.