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Fig. S6
Intron-retaining transcripts are enriched in the brain. Whole-mount RNAscope in situ hybridization assays showing the expression pattern of intron-retaining transcripts. (A, A') odc1, as a positive control, was broadly expressed in both siblings (A) and fn10a mutants (A'). (B, B') dapB, as a negative control, had little or no signal, except in the ear and notochord region, in siblings (B) and mutants (B'). (C-C', D-D') Intron-containing probes for ccnb1 and tardbp were enriched in the brain of fn10a mutants (C', D'; arrows) compared with siblings (C, D). Scale bars, 400 μm; n ≥10 for each panel.
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