ZFIN Image: Mu et al., 2017, Fig. 3

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Figure Caption

Fig. 3

Succinate promotes vessel formation in the zebrafish embryo. A. Effect of succinate on angiogenesis was evaluated in zebrafish embryos. Transgenic (flk1: GFP) zebrafish embryos were picked out and distributed into a 24-well microplate (9 embryos/well), and pre-treated with 0.2 μg/mL PTK787 (VEGFR inhibitor, Sigma). Then, various concentration of succinate (0, 100, 200, 400, 800 μM) or 10 ng/mL of VEGF₁₆₅ were added to the culture for twenty-four hours. Representative photographs are listed to show the subintestinal vessels (SIVs) indicated by white arrow. B, C. Quantitative analysis of the overall length of zebrafish SIVs are shown. Data are presented as mean ±SD.*p<0.05 and **p<0.01 versus PBS control.

Acknowledgments

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