|
Fig. 9
Analysis of vascular marker expression in vegfaa mutant embryos. (A-P) In situ hybridization analysis of arterial marker cldn5b (A,B), ephb2a (C,D), notch3 (E,F), pan-endothelial marker etv2 (G,H) and venous marker stab1l (I,J), dab2 (K,L), lyve1b (M,N), and mrc1a (O,P) expression in vegfaa-/- and wild-type sibling embryos at 24 hpf. Note the absence of arterial marker expression in vegfaa mutants (B,D,F) while venous markers are expanded into the arterial region of the single vessel present in vegfaa mutants (J,L,N,P). etv2 expression is reduced in vegfaa mutants and intersegmental vessels are absent due to the failure of angiogenesis (G,H). (Q) qPCR analysis of vascular marker expression in vegfaa-/- embryos compared to their siblings at 24 hpf. Note that all arterial and pan-endothelial markers are strongly downregulated, while flt4 and stab1l are only mildly downregulated. In contrast, venous-specific dab2 and lyve1b expression is increased, while mrc1a expression is unchanged. * p<0.05; ** p<0.01, ±SEM is shown.
Reprinted from Developmental Biology, 424(2), Chetty, S.C., Rost, M.S., Enriquez, J.R., Schumacher, J.A., Baltrunaite, K., Rossi, A., Stainier, D.Y., Sumanas, S., Vegf Signaling Promotes Vascular Endothelial Differentiation by Modulating etv2 Expression, 147-161, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.